Nematode 20 kD Lipid-binding proteins (LBP-20)
These proteins, initially identified as surface antigens of Onchocerca volvulus, have been identified in many nematode species from within the Rhabditida-Secernentea. Caenorhabditis elegans has six LBP-20 homologues.
Below is the alignment of nematode LBP-20 homologues used to build the phylogenetic tree in Figure 3C of Blaxter "C. elegans is a nematode". The alignment is in NEXUS format, and also includes, in addition to the sequences presented in Fig 3C, LBP-20 homologues identified from other filarial nematodes (Loa loa, Acanthocheilonema viteae) and a second partial LBP-20 EST fragment from Pristionchus pacificus.
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#NEXUS [nematode LBP-20 proteins] [Mark Blaxter, 27/07/98] BEGIN DATA [block-name] ; DIMENSIONS NTAX=15 NCHAR=212; FORMAT MISSING=? INTERLEAVE DATATYPE = PROTEIN GAP=- ; OPTIONS GAPMODE = NEWSTATE ; MATRIX [N-glycosylation sites nis N-LT NKS ] B_malayi_LBP_20 MYHRLILLA-LVGTTMANVIP-----FSMSNIPEEYKEFIPEEVRNFYKD-LTVEDKEILRELASKHA-TFANEDAALEALKDKSDKLYKNAVELRNFVK A_viteae_LBP_20 ????????????????ANVVP-----FPMSNIPEEYKEFMPEEVKNFYKN-LSIFLCQILRELASKHA-TFTNEDAALEALKNKSDKLYQKAVELRNLVK O_volvulus_LBP_20 MYHQLILMA-LIGVIMANVVP-----FSMSNIPEEYKEFIPEEVKNFYKN-LTQEDRQILRELASKHA-TFTNEDAALEALKNKSDKLYQKAVELRNFVK L_loa_LBP_20 MYHQLILLA-LIGTIMANVIP-----FSLSNISEEYKEFIPEEVRNFYKG-LTAEDKEILRDLASKHA-TFANEDAALEALKDKSDKLYKNAVELRNFVE G_pallida_SEC_1 MQRLLLCLTGASFIVLLFGAS--LPPIDISSIPEQYRELIPKEVIDFYNT-LTAEDKQALKEVAERHE-EFQTEEQAMEALKAKSEKLHSKAVELRNLVK N_americanus_LBP_20 MIRQIALIV--LLFTQFLLVP----AFKYEDIPADYRDLMPPEARDFLQN-LSDGDKTVLKEVFKAGP-YKNTEE-SIAALKKKSPELGAKVEKLHAMVK Ce_F15B9_1 MSKLLLLVLSLLFFITSAFPFG----EPQAGGFQKFKNLLPRELVEAYSN-LSQKDQPDLKDVFRNHQ-NYRNEQEMVNALKMKNPALGARMERRLMALK Ce_F15B9_2 MSRLFAFNVFCLVLLRFSAAAPADDSSPFSQILKQHKDLLPSEVVQAYQD-LSPEEKAALKDVFKNYK-SYKNEGELIAALKEKSSSLGEKAEKLQAKLQ Ce_F15B9_3a MLLRFFAILLIFHFSFAADG-------IFEAVIESYKDALPPKVVAAFDN-LSPGESAIMKEVFMNYD-KFTSIADLIVAIKKKSESLGSFFEKLYIEID Ce_F02A9_2 MIRATIILAAVAALAFSAPVP--EVPENYDDIPAEYKSLIPAEVSEHLKS-ITPEEKAILKEVAKGYK-DFKSEDDFLNALKEKSPTLHEKASKLHQIVK Ce_F02A9_3 MIRAFLVVALASVAVFSAPIP--EVPQNFDDIPAEYKGLIPAEVAEHLKA-ITAEEKAALKELAQNHK-EYKTEEEFKAALKEKSPSLYEKAGKLEALLT Ce_W02A9_e MIRIFLVIALASVAVFSAPIS--RLPQSLDDIPAEFKELIPAKVTEYLKS-ITTEEKAAIKEFIKSVMGGNKSVEELSADIKERSPSLYAKVEKLDVLLR C_briggsae_EST MIRATIVFACIGAVAFSAPIP--EVPENYEDIPAEYKSLIPAEVAEHLKS-ITPEEKAILKDVAKGYK-DFKSEDDFLNALKEKSPALHEKASKLHQIVK Pristionchus_EST1 MIRGLALLALVAACAFXAPF------NSIDDVPAEYKDLIPAQAKEFLTG-XTDEDKKVLKEIAANYA-SYKNEDEALNALKEKSPALYEKAEKLGAYLK Pristionchus_EST2 SAPFNSIDDVPDXVQGXDPR----AGKEFLTSXTDEDKKIXKEVAMNYKEYKTEEDALNALKEKSPA--LYEKAQKLGNYLKEKLDALNPEAKKFAEEIF [glycosylation sites NTT] B_malayi_LBP_20 AKIDSLK-PDAKIFVDEIIAKAR---SLRSDDGHKLDTEKIKQAARDIIAKYQALSEETKEELKVTFPAIAKIIGNEKLKRNASTFLQKN?????????????????????? A_viteae_LBP_20 AKIDSLK-PDAKAFVDEIIAKVR---SLRPEDGQKLDVEKLKQAARDIIAKYEALNEETKEELKAPFPNTTKIITNEKFTRIANSFLQKN?????????????????????? O_volvulus_LBP_20 AKIDSLK-PDAKAFVDEIIAKVR---SLRPEDGQKLDMEKLKQAARDIIAKYEALNEETKEELKATFPNTTKIITNEKFKRIANSFLQKN?????????????????????? L_loa_LBP_20 ???????????????????????????????????????????????????????????????????????????????????????????????????????????????? G_pallida_SEC_1 EKIDKLV-PGAKTFVTETIEKLK---AMRPKSGEKPNLEELRKGANDTIEKFKALSVEAKESLKANFPKITGVIQSEKFQALAKSLLKTEGAAPAA???????????????? N_americanus_LBP_20 SKIAALG-PEAKGFAEKSIEIAR-GIKARYYTGNEPTKDDLKASVKEVLKLYKAMSDAGKADFGKQFPFLAK???????????????????????????????????????? Ce_F15B9_1 QKIDGLSSEEAKGFIQNLISTGR-QIYAQRLNGQQMDQSQLRQVGMGIAMHYRSLPPYAQQELQSTFPQIFQFMRQMREQRLRSMMGGFFGGGGGIGMGQGMGQGMGMGMGK Ce_F15B9_2 KKVDALS-PKPKDFVNELIAGGR-GLYARSVNGEKISVSEIKLLIETQVAAYKALPAEAQDELKKNFGGVAKFLEDDKTQTLIAKLLEKNNNQ??????????????????? Ce_F15B9_3a AEIQALT-PETKKFVTEMLGIGR-AIYTAQIVGIPLDSKEVLPVFAKQFTSFKSLSDATKEELEKTFLGLYKFASNDKIKTEIDKLL????????????????????????? Ce_F02A9_2 DKVNALN-DEAKAFVKKAIAEGR-KIHAQYLAGEKPSLDTLKTTAKTHIEAYKGLSQDAKDSIAKEFPILTGFFKNEKVQAMVGQYLN???????????????????????? Ce_F02A9_3 AKFEKLD-ATAQALVKKIIAKGR-ELHQQYLAGDKPTLDSLKELAKGYIAEYKALSDDAKATITAEFPILTGFFQNEKIQAIVGQYVN???????????????????????? Ce_W02A9_e TKLAKLD-PAALALFGKVIAQGL-SFRQQFHAGYQPTPEMVKKLFKGYIAEYKTLSENAKATITDEFPIVVEFFQHEKIQAIIQQIVNY??????????????????????? C_briggsae_EST DKVNSLN-DEAKAFVKKAIAERDGKIHASILGLGPILTGFFKNEKVQAMVGQYIN????????????????????????????????????????????????????????? Pristionchus_EST1 EKLDALN-PEAKKFAEEIFGDARKIQAQVVAGN-KPSIADQDKVIE-IVGKFEALSAEAKATIEKQFPITASIVKNEKXQELYKKFVTKEN????????????????????? Pristionchus_EST2 GDARKIQAAVIAGNRPSLDELKAK???????????????????????????????????????????????????????????????????????????????????????? ; ENDBLOCK;