Brugia malayi GSS BAC End Sequences

An initial set of BAC end sequences was generated from clones identified by hybridisation ,and randomly selected clones. They are tabulated here with a listing of the hits found by BLAST search to bacterial, filarial and general databases. The linked blast* items will take you to a blast output for the relevant search. The AT content is tabulated for information: Brugia's genome is 75% AT.

David Guiliano and Mark Blaxter

BAC number

Sequencing Primer

Identification

Hits to Bacterial

Hits to Filarial

blastx search

blastn vs dbest

blastn vs nr

AT Content

O3-2

T7

none

none

none

blastx

blastn vs dbest

blastn vs nr

78%

O3-2

SP6

none

none

MBAFCF1A10T3

Novel

blastx

blastn vs dbest

blastn vs nr

65%

P19-1

T7

none

none

none

blastx

blastn vs dbest

blastn vs nr

67%

P19-1

SP6

none

none

none

blastx

blastn vs dbest

blastn vs nr

65%

A6-8

T7

none

none

none

blastx

blastn vs dbest

blastn vs nr

70%

BAC-3-1

T7

the sequence is all vector!

vector

none

blastx

blastn vs dbest

blastn vs nr

not counted

BAC-3-2

T7

the sequence is all vector!

vector

none

blastx

blastn vs dbest

blastn vs nr

not counted

BAC-4-2

T7

the sequence is all vector!

vector

none

blastx

blastn vs dbest

blastn vs nr

not counted