National Center for Biotechnology Information (NCBI)
welcome to the blast2 network service.
PEPTIDE SEQUENCE DATABASES
nr Non-redundant GenBank CDS translations+PDB+SwissProt+PIR
month All new or revised GenBank CDS translation+PDB+SwissProt+PIR
sequences released in the last 30 days
pdb PDB protein sequences
yeast Yeast (Saccharomyces cerevisiae) protein sequences.
kabat Kabat Sequences of Proteins of Immunological Interest
alu * Translations of Select Alu Repeats from REPBASE
swissprot SwissProt sequences
NUCLEOTIDE SEQUENCE DATABASES
nr Non-redundant GenBank+EMBL+DDBJ+PDB sequences
(but no EST, STS, GSS, or HTGS sequences)
month All new or revised GenBank+EMBL+DDBJ+PDB sequences released in
the last 30 days
yeast Yeast (Saccharomyces cerevisiae) genomic nucleotide sequences.
est + Non-redundant Database of GenBank+EMBL+DDBJ EST Division
sts + Non-redundant Database of GenBank+EMBL+DDBJ STS Division
htgs High Throughput Genomic Sequences
pdb PDB nucleotide sequences
vector Vector subset of GenBank
mito * Database of mitochondrial sequences, Rel. 1.0, July 1995
gss Genome Survey Sequences (includes single-pass genomic data, exon-
trapped sequences, and Alu PCR primers.
kabat Kabat Sequences of Nucleic Acid of Immunological Interest
epd Eukaryotic Promotor Database
alu *+ Select Alu Repeats from REPBASE
BLASTX 1.4.11 [24-Nov-97] [Build 24-Nov-97]
Reference: Gish, Warren and David J. States (1993). Identification of
protein coding regions by database similarity search. Nat. Genet. 3:266-72.
Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, and David J.
Lipman (1990). Basic local alignment search tool. J. Mol. Biol. 215:403-10.
Notice: statistical significance is estimated under the assumption that
the equivalent of one entire reading frame in the query sequence codes for
protein and that significant alignments will involve only coding reading
frames.
Query= tmpseq_1
(366 letters)
Translating both strands of query sequence in all 6 reading frames
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
329,726 sequences; 100,504,245 total letters.
Searching..................................................done
Smallest
Sum
Reading High Probability
Sequences producing High-scoring Segment Pairs: Frame Score P(N) N
gi|643453 (U15516) PPmABPXM precursor [Staphylo... +1 61 0.998 1
gi|551988 (M91199) BBXM [Staphylococcus xylosus] +1 61 0.998 1
gi|2342750 (U38693) recombinant heterologous sur... +1 61 0.998 1
gi|2431891 (AF013699) NADH dehydrogenase [Drymon... +2 51 0.9994 2
gnl|PID|e1323171 (Z82070) W04E12.2 [Caenorhabditis ele... -2 60 0.9998 1
gi|643453 (U15516) PPmABPXM precursor [Staphylococcus carnosus]
Length = 666
Plus Strand HSPs:
Score = 61 (27.9 bits), Expect = 6.1, P = 1.0
Identities = 12/20 (60%), Positives = 13/20 (65%), Frame = +1
Query: 1 PLESTCRHASFKTHQKIRKD 60
PLESTCRHAS K + D
Sbjct: 252 PLESTCRHASLDALAKAKAD 271
gi|551988 (M91199) BBXM [Staphylococcus xylosus]
Length = 492
Plus Strand HSPs:
Score = 61 (27.9 bits), Expect = 6.1, P = 1.0
Identities = 11/11 (100%), Positives = 11/11 (100%), Frame = +1
Query: 1 PLESTCRHASF 33
PLESTCRHASF
Sbjct: 280 PLESTCRHASF 290
gi|2342750 (U38693) recombinant heterologous surface receptor [shuttle
vector pSEmp18BBXM]
Length = 468
Plus Strand HSPs:
Score = 61 (27.9 bits), Expect = 6.1, P = 1.0
Identities = 12/20 (60%), Positives = 13/20 (65%), Frame = +1
Query: 1 PLESTCRHASFKTHQKIRKD 60
PLESTCRHAS K + D
Sbjct: 54 PLESTCRHASLDALAKAKAD 73
gi|2431891 (AF013699) NADH dehydrogenase [Drymonia urceolata]
Length = 684
Plus Strand HSPs:
Score = 51 (23.4 bits), Expect = 7.5, Sum P(2) = 1.0
Identities = 11/38 (28%), Positives = 21/38 (55%), Frame = +2
Query: 50 FGRTFKLFKRTWIIMLIMDMMPVSTFLS*FEIQLLSQN 163
F K+F+R W ++ I+ + V F + IQL++ +
Sbjct: 21 FPTATKIFRRMWALVSILLLSIVMIFSTNLSIQLINNS 58
Score = 45 (20.6 bits), Expect = 7.5, Sum P(2) = 1.0
Identities = 8/19 (42%), Positives = 13/19 (68%), Frame = +2
Query: 257 MMISLKYFGMNICIYLY*P 313
+ +S+ YFG+ I +LY P
Sbjct: 595 LSVSIAYFGIFIASFLYKP 613
gnl|PID|e1323171 (Z82070) W04E12.2 [Caenorhabditis elegans]
Length = 199
Minus Strand HSPs:
Score = 60 (27.5 bits), Expect = 8.4, P = 1.0
Identities = 15/47 (31%), Positives = 26/47 (55%), Frame = -2
Query: 308 NTDIYKYSSQNILNLSSFGVVAVNLK**DSISFL*LKXYSVN*IWFT 168
N + K S+ N+L L++FG+ V L+ + FL + +V WF+
Sbjct: 18 NPVVRKLSTVNVLALTAFGLSMVILRDVFELDFLLMNTVAVTVAWFS 64
Parameters:
V=5
Lambda K H
0.318 0.135 0.401
Cutoff to enter 2nd pass: >= 45 ( 0.0 bits)
E S T1 T2 X1 X2 W Gap
10.0 62 12 12 -16 -22 40 50
Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR
Posted date: Oct 14, 1998 7:57 AM
# of letters in database: 100,504,245
# of sequences in database: 329,726
Number of Hits to DB: 1st pass: 59881388, 2nd pass: 314182
Number of Sequences: 1st pass: 329726, 2nd pass: 637
Number of extensions: 1st pass: 978900, 2nd pass: 260179
Number of successful extensions: 1st pass: 637, 2nd pass: 1632
Number of sequences better than 10: 5