National Center for Biotechnology Information (NCBI)
welcome to the blast2 network service.
PEPTIDE SEQUENCE DATABASES
nr Non-redundant GenBank CDS translations+PDB+SwissProt+PIR
month All new or revised GenBank CDS translation+PDB+SwissProt+PIR
sequences released in the last 30 days
pdb PDB protein sequences
yeast Yeast (Saccharomyces cerevisiae) protein sequences.
kabat Kabat Sequences of Proteins of Immunological Interest
alu * Translations of Select Alu Repeats from REPBASE
swissprot SwissProt sequences
NUCLEOTIDE SEQUENCE DATABASES
nr Non-redundant GenBank+EMBL+DDBJ+PDB sequences
(but no EST, STS, GSS, or HTGS sequences)
month All new or revised GenBank+EMBL+DDBJ+PDB sequences released in
the last 30 days
yeast Yeast (Saccharomyces cerevisiae) genomic nucleotide sequences.
est + Non-redundant Database of GenBank+EMBL+DDBJ EST Division
sts + Non-redundant Database of GenBank+EMBL+DDBJ STS Division
htgs High Throughput Genomic Sequences
pdb PDB nucleotide sequences
vector Vector subset of GenBank
mito * Database of mitochondrial sequences, Rel. 1.0, July 1995
gss Genome Survey Sequences (includes single-pass genomic data, exon-
trapped sequences, and Alu PCR primers.
kabat Kabat Sequences of Nucleic Acid of Immunological Interest
epd Eukaryotic Promotor Database
alu *+ Select Alu Repeats from REPBASE
BLASTX 1.4.11 [24-Nov-97] [Build 24-Nov-97]
Reference: Gish, Warren and David J. States (1993). Identification of
protein coding regions by database similarity search. Nat. Genet. 3:266-72.
Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, and David J.
Lipman (1990). Basic local alignment search tool. J. Mol. Biol. 215:403-10.
Notice: statistical significance is estimated under the assumption that
the equivalent of one entire reading frame in the query sequence codes for
protein and that significant alignments will involve only coding reading
frames.
Query= tmpseq_1
(435 letters)
Translating both strands of query sequence in all 6 reading frames
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
322,347 sequences; 97,848,901 total letters.
Searching..................................................done
Smallest
Sum
Reading High Probability
Sequences producing High-scoring Segment Pairs: Frame Score P(N) N
gi|2338552 (AF013277) topoisomerase II [Bomb... -1 63 0.98 1
pir||S48472 probable membrane protein YIL100c... +1 57 0.998 2
sp|Q04991|YM68_YEAST HYPOTHETICAL 56.2 KD PROTEIN IN E... +1 56 0.999 2
gi|2338552 (AF013277) topoisomerase II [Bombyx mori]
Length = 1547
Minus Strand HSPs:
Score = 63 (28.9 bits), Expect = 4.2, P = 0.98
Identities = 19/66 (28%), Positives = 32/66 (48%), Frame = -1
Query: 390 TFLDXXEESLXLRYYFSLESNLTTKIYMQH*SLEMFNYGQDNKLVN*IVRIDIICRVXLN 211
T L ++S RY F+L S LT I+ H + + +DN+ + + + II V +N
Sbjct: 800 TRLSGGKDSASPRYIFTLMSPLTRLIFHPHDDPLLVHEFEDNQKIEPVYYLPIIPMVLVN 859
Query: 210 TEKSYG 193
+ G
Sbjct: 860 GVEGIG 865
pir||S48472 probable membrane protein YIL100c-a - yeast (Saccharomyces
cerevisiae) gi|558706 (Z38125) orf, len: 112, CAI: 0.07, overlapped
by orf 36949..36826 qv [Saccharomyces cerevisiae]
Length = 112
Plus Strand HSPs:
Score = 32 (14.7 bits), Expect = 6.5, Sum P(2) = 1.0
Identities = 5/7 (71%), Positives = 5/7 (71%), Frame = +2
Query: 44 YGIHTYC 64
Y IH YC
Sbjct: 26 YHIHCYC 32
Score = 57 (26.1 bits), Expect = 6.5, Sum P(2) = 1.0
Identities = 15/30 (50%), Positives = 16/30 (53%), Frame = +1
Query: 52 PYLLSYLLIAIELSLKLSHYNKKIYIYIYI 141
P LLS LL L N K+YIYIYI
Sbjct: 35 PLLLSLLLHYTVLFSPFGARNHKLYIYIYI 64
sp|Q04991|YM68_YEAST HYPOTHETICAL 56.2 KD PROTEIN IN ERG8-UBP8
INTERGENIC REGION pir||S57589 probable membrane protein YMR221c -
yeast (Saccharomyces cerevisiae) gi|887602 (Z49939) unknown
[Saccharomyces cerevisiae]
Length = 504
Plus Strand HSPs:
Score = 56 (25.7 bits), Expect = 6.9, Sum P(2) = 1.0
Identities = 9/15 (60%), Positives = 12/15 (80%), Frame = +1
Query: 40 SLWHPYLLSYLLIAI 84
SLW PYL+ Y L+A+
Sbjct: 116 SLWDPYLVGYTLLAV 130
Score = 41 (18.8 bits), Expect = 6.9, Sum P(2) = 1.0
Identities = 10/21 (47%), Positives = 14/21 (66%), Frame = +1
Query: 220 HTTNNVYPDNLIH*LVILTII 282
HTT N+ P + LVILT++
Sbjct: 454 HTTFNMNPFPINLTLVILTVV 474
Parameters:
V=5
Lambda K H
0.318 0.135 0.401
Cutoff to enter 2nd pass: >= 46 ( 0.0 bits)
E S T1 T2 X1 X2 W Gap
10.0 62 12 12 -16 -22 40 50
Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR
Posted date: Aug 24, 1998 6:54 AM
# of letters in database: 97,848,901
# of sequences in database: 322,347
Number of Hits to DB: 1st pass: 67035514, 2nd pass: 378340
Number of Sequences: 1st pass: 322347, 2nd pass: 583
Number of extensions: 1st pass: 1214091, 2nd pass: 312370
Number of successful extensions: 1st pass: 583, 2nd pass: 3252
Number of sequences better than 10: 3