Einburgh BAC-EST Hybridization ResultsEinburgh BAC-EST Hybridization ResultsThe table below shows the results of hybridizations of ESTs to the gridded BAC library in Edinburgh. This page will be udated as more are carried out. PCR verification of positive BACs was performed with primers shown to amplify genomic fragments from B. malayi total gDNA .
David Guiliano
|
Probe (Probe Source) |
Hybribizing BACs |
Comments |
|
rpL26 (MB4SLM1C1T3) |
L22-2 B16-5 L6-8 |
|
|
SNRP (MBAFCX0CE11T3) |
B6-8 A5-10 |
PCR positive PCR positive |
|
Fibrillarin (MBAFCX3C04T3) |
C18-9 O22-12 |
PCR positive PCR positive |
|
MIF-1 (MB2SLJ1E04T3) |
O3-2 P19-1 A6-8 |
In Preparation for Sanger In Preparation for Sanger In Preparation for Sanger |
|
rpP0 (MBL2SJ2G8T3) |
None |
|
|
rpS16 (MBL2SJ9A6T3) |
None |
|
|
NDK (MB4SLY1D09T3) |
D4-11 N16-4 N20-6 P5-12 D4-5 N19-6 |
|
|
Proteosome Zeta Chain (MBAFCCZ4E08T3) |
N4-10 |
|
|
rDNA cluster (Brugia malayi 18S 1.5kB fragment) |
many clones |
|
|
Hha-1 Repeat (biotin labeled oligo probe provided by NEB) |
many clones |
|