A hybrid mapping proposal for the Brugia genome.

1 Sample without replacement of the complete library

• carried out in Edinburgh

2 EST hybridisation

• carried out in all WHO genome project labs
  Coordinated by Al Scott, Baltimore

These two STS content mapping approaches will be entirely complementary.

Additional Approaches

3 Telomeric cloning

• carried out in Edinburgh

This will be integrated into the BAC map by cloning-out of nonrepetitive telomere associated probes and positioning these on the BAC map.

4 Rare-cutter restiction mapping

• pulsed-field gel blots of Not I, Sfi I, or other rare-cutter genomic DNA digests will be prepared. These can be hybridised with contig end-probes to link contigs
• a "linking library of clones containing Not I or Sfi I sites will provide a means of assembling restriction fragments into larger units.