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Hedgehog consists of 96 pins inserted into a rigid-back plate
The pins in this hedgehog are of 2mm diameter, cut to length from stainless steel rods.
The positions of the 8 x 12 matrix(9 x 9 mm apart) are marked out in the centre of the back-plate.
The holes are drilled slightly smaller than the diameter of the pins.
The lengths of stainless steel for the pins are hammered into the pre-drilled array .
The handle is secured to the back-plate by small screws positioned between the pins
Colony Printing Jig Construction
The jig is constructed from a rigid perspex base plate on to which a box frame is attached.
The box frame is 1/2 a pin space (ie ~4.5mm) longer and wider than the hedgehog(ie frame 124x90mm):
this will allow four 96 colony arrays to be printed on a single membrane (see colony printing protocol).
The lower portion of one end of the box frame is open so that a membrane holding plate can be slid into the frame.
This plate is 86x128mm and slides into the box frame through a gap in the side wall between perpex runners.
A hole is drilled in the opposite end into which a rod is inserted to eject the membrane holding plate after printing is completed.