|
|
|
|
570 580 590 600 610 620
TAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAAT
V G P N E W D R R Q L V V A L S R A Y Y
_________________________LAC Z ORF__________________________
____________________________MCS_____________________________
___M13 -20 PR____
___
________
_____M13 L PRIMER_20____
_________M13R BY M13L PRODUCT; 275 BASES IN WT__50_______
Xho_1
|
630 640 650 660 670 680
ACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATA
S E S Y P S N P V P G G R S T S P I S L
_________________________LAC Z ORF__________________________
____________________________MCS_____________________________
_____10_______
___T7 PLUS LONG __20
10________20__
_60________M13R BY M13L PRODUCT; 275 BASES IN WT__110_______
___XHO I / ECO R I_0__
_______
_______
Eco_R_1 Xba_I
| |
690 700| 710 720 730| 740
AGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCG
S S I S N R C G P P D V L E L A A A V A
_________________________LAC Z ORF__________________________
____________________________MCS_____________________________
120________M13R BY M13L PRODUCT; 275 BASES IN WT__170_______
___XHO I / EC____
_10________20
_10________20________ECO-SAC FRAGMENT____50________60_______
________1_ECO-XBA FRAGMENT__30_______
___SK PRIMER_____
20_SK PLUS
LONG ____
750 760 770 780 790 800
CGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCA
T S S W S K N G K T L T L Q A S P T I M
_________________________LAC Z ORF__________________________
____________________________MCS_____________________________
180________M13R BY M13L PRODUCT; 275 BASES IN WT__230_______
____T3 PRIMER______
___SAC PRIMER____
_70_ECO-SAC FRAGMENT_90__
__
810 820 830 840 850 860
TGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGA
T M
_______
____________________________MCS_____________________________
24_M13R BY M13L PRODUCT; 275 BASES____
___2_M13 R PRIMER_______
___10_________
870 880 890 900
GCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCT
_____________MCS_______________