Genes
expressed in Brugia malayi infective third stage larvaeGenes
expressed in Brugia malayi infective third stage larvaeInstitute of Cell, Animal and Population Biology,
University of Edinburgh, EH9 3JT UK
Department of Molecular Microbiology and Immunology, School of
Hygiene and Public Health, Johns Hopkins University, Baltimore,
MD 21205 USA
Department of Biological Sciences, Clark Science Center, Smith
College, Northampton, MA 01063 USA
New England Biolabs, Inc., 32 Tozer Road, Beverly, MA 01915-5599
USA
copyright of the article © 1996 rests with Elsevier Science
Abstract
We have used a tag sequencing approach to survey genes expressed in
the third stage infective larvae of the human filarial nematode
parasite Brugia malayi. RNA was isolated from late
vector-stage L3 larvae after day nine or ten of infection in
mosquitos, and converted to cDNA by reverse transcriptase.
Double-stranded cDNA was produced by either conventional methods
(non-SL cDNA library) or by PCR using the nematode spliced leader
(SL1) and oligo d(T) primers (SL cDNA library). Two clone libraries
(one from SL and one from non-SL cDNAs) were constructed in lambda
ZapII. A set of these full-length clones was selected and 596 inserts
were sequenced from the 5' end. We have identified 364 B.
malayi genes (the majority of which are new) that encode
housekeeping proteins, structural proteins, proteins of immediate
immunological or drug-discovery interest as well as a large class of
novel sequences which may prove to have significant involvement in
host invasion. Extensive, genome-wide approaches to the analysis of
larval gene expression are now possible for B. malayi. We
present several examples of this approach.
The text of the paper is no longer available on the www (consult
MBP), but the basic data is in the following tables:
Table 1 overview of the L3
EST dataset
Table 2 database similarities
and so on of sequences