see here for more information on this library
FAX: 1 410 955 3442
TEL: 1 410 955 0105
EMAIL: ascott@hpcsun01.sph.jhu.edu.
LAB:Department of Immunology and Infectious Diseases
INSTITUTION: School of Hygiene and Public Health, The Johns Hopkins
University
ADDRESS: 615 N. Wolfe Street, Baltimore, Maryland 21205 USA
ORGANISM: Brugia malayi
ABBREVIATED NAME: B. malayi
STRAIN:TRS Labs
COMMON NAME: lymphatic filarial nematode
VECTOR: lambda ZapII
VECTOR TYPE: lambda phage
RESTRICTION ENZYME 1: XbaI
RESTRICTION ENZYME 2: EcoRI
HOST: XL1-Blue MRF-1
DETAILED DESCRIPTION: Full length cDNA was prepared by RT-PCR from
mRNA from vector-stage infective L3 larvae of the human filarial
nematode parasite Brugia malayi using nematode spliced leader (SL, 5'
end) and oligo-d(T) (3' end) primers. The library had an unamplified
titreof 1.6 x 10E6 per ml and 94% of clones have inserts (mean length
~700 bp).
AVAILABILITY: from Alan Scott
MEDLINE UID:
CITATION: Unpublished
STATUS: 1
Additional information
Please quote the ref. no JHU93SL-BmL3 in the source section of your Genbank submission form when you submit sequence data from this library.
You must quote the name " Brugia malayi infective L3 JHU93SL-BmL3" in the LIBRARY section of your dbest submission form when you submit est data from this library.
Vector: Lambda Zap II
Insert: Brugia malayi L2/L3 cDNA
Cloning sites: Xba I (5' end) and Eco RI (3' end)
Unamplified titer: 1 x 106 pfu/ml
Amplified titer: 7.2 x 109 pfu/ml
Background: < 6% non-recombinants
Average insert size: ~ 700 bp
Transcripts: 98 % full length transcripts
Primers used in library construction:
5' end: XSL: 5' GCT CTA GAG CGG TTT AAT TAC CCA AGT TTG AG 3' 3' end:
XET: 5' GCG CGC TCG AGA ATT CTT TTT TTT TTT TTT TTT TTT 3'
Host Strain Information:
The XL1- Blue MRF' strain is the recommended host strain for
amplification and screening of this Lambda Zap II cDNA library. The
XL1-Blue MRF' strain is a restriction minus strain recommended for
plating unamplified libraries, amplifying and screening libraries and
allows blue/white color selection of recombinant clones when grown on
plates containing IPTG and X-gal.
The bacterial stock is stored and streaked on LB-tet plates and grown
as O/N cultures in the absence of tetracycline.
Genotype:
D (mcrA) 183, D (mcrCB-hsdSMR-mrr) 173, end A1, supE44, thi-1, recA1,
gyrA96, relA1, lac, [F' proAB, lacIqD M15, Tn10 (tetr)]
Reference: Jerpseth, J. et al., (1992) Strategies 5(1).
Authors
Inca Ghosh, Nithyakalyani Raghavan, Ward Eisinger, Alan Scott
Dept. of Molecular Microbiology and Immunology
School of Hygiene and Public Health
Johns Hopkins University
615 N. Wolfe St.
Baltimore, MD 21205
Tel: (410) 955-3442
Fax: (410) 955-0105
nkr@helix.nih.gov
ascott@hpcsun01.sph.jhu.edu