Brugia malayi second stage mosquito-derived larval cDNA library JHU96SL-BmL2 from Alan Scott
FAX: 1 410 955 3442
TEL: 1 410 955 0105
EMAIL: ascott@hpcsun01.sph.jhu.edu.
LAB:Department of Immunology and Infectious Diseases
INSTITUTION: School of Hygiene and Public Health, The Johns Hopkins
University
ADDRESS: 615 N. Wolfe Street, Baltimore, Maryland 21205 USA
ORGANISM: Brugia malayi
ABBREVIATED NAME: B. malayi
STRAIN:TRS Labs
COMMON NAME: lymphatic filarial nematode parasite of humans
VECTOR: lambda UniZap
VECTOR TYPE: Phage
RESTRICTION ENZYME 1: RESTRICTION ENZYME 2:
HOST:
DETAILED DESCRIPTION:
AVAILABILITY: .
MEDLINE UID:
CITATION: Genes expressed in second stage larval Brugia malayi
STATUS: Unpublished (1996)
further information
L2 B. malayi SL cDNA Library
Please quote the ref. no JHU96SL-BmL2 in the source section of your Genbank submission form when you submit sequence data from this library. The library is available through the Filarial Genome Center at Smith College.
Vector: Uni-Zap XR
Insert: Brugia malayi L2 cDNA
Cloning sites: Xho I (5' end) and Eco RI (3' end)
Unamplified titer: 1 x 10^6 pfu/ml
Amplified titer: 2 x 10^9 pfu/ml
Background: < 4% non-recombinants
Primers used in library construction:
5' end:
ESL: 5' CCG GAA TTC CGG GGT TTA ATT ACC CAA GTT TGA G 3' Eco RI spliced leader sequence 3' end: XT: 5' GCG CGC TCG AGC GTT TTT TTT TTT TTT TTT TTT 3' Xho I
Host Strain Information:
The XL1- Blue MRF' strain is the recommended host strain for amplification and screening of this Uni-Zap cDNA library. The XL1-Blue MRF' strain is a restriction minus strain recommended for plating unamplified libraries, amplifying and screening libraries and allows blue/white color selection of recombinant clones when grown on plates containing IPTG and X-gal.
The bacterial stock is stored and streaked on LB-tet plates and grown as O/N cultures in the absence of tetracycline.
Genotype:
D (mcrA) 183, D (mcrCB-hsdSMR-mrr) 173, end A1, supE44, thi-1, recA1, gyrA96, relA1, lac, [F' proAB, lacIqD M15, Tn10 (tetr)]
Reference: Jerpseth, J. et al., (1992) Strategies 5(1).
Acknowledgments:
Nithyakalyani Raghavan and Alan Scott
Dept. of Molecular Microbiology and Immunology
School of Hygiene and Public Health
Johns Hopkins University
615 N. Wolfe St.
Baltimore, MD 21205
Tel: (410) 955-3442 Fax: (410) 955-0105