Genome Project cDNA Libraries.

Seven libraries have been constructed and are actively being sequenced (RED arrows above): these are described below (more details, including descriptions of other libraries are here).

Two new libraries have just been constructed (PINK arrows above) and will be available later this year.

Stage

Library Name

Vector

Cloning Sites

Construction Details

L1 = Mf

SAW94LS-BmMF

lambda UniZap XR

EcoR I - Xho I

conventional dT primed cDNA

L2

JHU96SL-BmL2

lambda ZapII

EcoR I - Xho I

cDNA from SL-dT PCR of total first strand cDNA

L3 infective

JHU93SL-BmL3

lambda ZapII

Xba I - Eco R I

cDNA from SL-dT PCR of total first strand cDNA

L3 infective

SAW94WL-BmL3

lambda UniZap XR

EcoR I - Xho I

conventional dT primed cDNA

L3-L4 day 6-7

SAW97MLW-BML3D6

lambda UniZap XR

EcoR I - Xho I

conventional dT primed cDNA

L3-L4 day 8-9

under construction

lambda UniZap XR

EcoR I - Xho I

conventional dT primed cDNA

L4

JHU93SL-BmL4

lambda ZapII

Xba I - Eco R I

cDNA from SL-dT PCR of total first strand cDNA

Adult Female

SAW94NL-BmAM

lambda UniZap XR

EcoR I - Xho I

conventional dT primed cDNA

Adult Male

SAW96MLW-BmAF

lambda UniZap XR

EcoR I - Xho I

conventional dT primed cDNA