|
|
Seven libraries have been constructed and are actively being sequenced (RED arrows above): these are described below (more details, including descriptions of other libraries are here).
Two new libraries have just been constructed (PINK arrows above) and will be available later this year.
|
Stage |
Library Name |
Vector |
Cloning Sites |
Construction Details |
|
L1 = Mf |
lambda UniZap XR |
EcoR I - Xho I |
conventional dT primed cDNA |
|
|
L2 |
lambda ZapII |
EcoR I - Xho I |
cDNA from SL-dT PCR of total first strand cDNA |
|
|
L3 infective |
lambda ZapII |
Xba I - Eco R I |
cDNA from SL-dT PCR of total first strand cDNA |
|
|
L3 infective |
lambda UniZap XR |
EcoR I - Xho I |
conventional dT primed cDNA |
|
|
L3-L4 day 6-7 |
SAW97MLW-BML3D6 |
lambda UniZap XR |
EcoR I - Xho I |
conventional dT primed cDNA |
|
L3-L4 day 8-9 |
under construction |
lambda UniZap XR |
EcoR I - Xho I |
conventional dT primed cDNA |
|
L4 |
lambda ZapII |
Xba I - Eco R I |
cDNA from SL-dT PCR of total first strand cDNA |
|
|
Adult Female |
lambda UniZap XR |
EcoR I - Xho I |
conventional dT primed cDNA |
|
|
Adult Male |
lambda UniZap XR |
EcoR I - Xho I |
conventional dT primed cDNA |