* Vector: Uni-Zap XR
* Insert: Wuchereria bancrofti adult male cDNA
* Cloning sites: Xba I (5' end) and Eco RI (3' end)
* Unamplified titer:2.5 x 105 pfu/ml
* Amplified titer: 1.2 x 109 pfu/ml
* Background: <10% non-recombinants
* Average insert size: ~ 600 bp
Primers used in library construction:
5' end: XSL: 5'GCT CTA GAG CGG TTT AAT TAC CCA AGT TTG AG 3'
3' end: XET: 5'GCG CGC TCG AGA ATT CTT TTT TTT TTT TTT TTTTTT 3'
Host Strain Information:
The XL1- Blue MRF' strain is the recommended host strain for
amplification and screening of this Lambda Zap II cDNA library. The XL1-Blue
MRF' strain is a restriction minus strain recommended for plating unamplified
libraries, amplifying and screening libraries and allows blue/white color
selection of recombinant clones when grown on plates containing IPTG and
X-gal.
NOTE: The bacterial stock is stored and streaked on LB-tet plates and grown as O/N cultures in the absence of tetracycline.
Reference: Jerpseth, J. et al., (1992) Strategies 5 (1).
Acknowledgments:
Inca Ghosh, Nithyakalyani Raghavan, Ward Eisinger, Alan Scott
Dept. of Molecular Microbiology and Immunology
School of Hygiene and Public Health
Johns Hopkins University
615 N. Wolfe St., Baltimore, MD 21205
Tel: (410) 955-3442 || Fax: (410) 955-0105
To request for libraries, please e-mail to:
(Nithyakalyani Raghavan) || (Alan L. Scott)