PCR amplification products from formalin fixed C. elegans after incubation in GTES (see text). Primer sets used were as follows: lane 1- A/26R, lane 2- 22F/DR, lane 3- DF/18p, lane 4 A/26R (positive control; amplification from fresh C. elegans), lane 5- 22F/26R, lane 6- A/22R, lane 7- A/9R, and lane 8- DF/DR. Product sizes are as indicated by marker. The PCR product in lane 3 was sequenced using the primer DF; no significant differences from the published C. elegans SSUrDNA sequence were observed. Cycling parameters, primer details and primer positions are in Appendix A1.