Developmental Biology 3 Practical

A mutant zoo

strain	locus	allele	chromosome	description
CB2	vab-1	e2	II	"variably abnormal": adult hermaphrodite head has a dorsal notch; incompleteley penetrant
CB66	unc-22	e66	IV	"uncoordinated"; nematodes twitch while moving; due to a defect in a muscle filament protein
CB189	unc-32	e189	III	"uncoordinated": nematodes coil ventrally on backing up; due to miswiring of the motor nervous system
CB14	dpy-6	e14	X	"dumpy"; nematodes are much shorter and fatter than wildtype
CB518	bli-5	e518	III	"blistered"; adults have cuticle blisters, especially on head; due to breakdown between layers of the cuticle; variable penetrance (more prevalent in older nematodes)
DA650	bor-1	g320	X	"bordering"; nematodes preferentially accumulate on the border between food and no food, also clump together; a mutant revealing a high degree of central nervous system integration
UL48	N2; glp-1::lacZ, rol-6		extrachromosomal element	"roller"; these nematodes are transgenic and carry an artificially introduced rol-6 mutant allele which causes them to have a twisted cuticle and thus to roll; the glp-1::lacZ means they also carry a marker enzyme gene under the control of the promoter of the glp-1 gene.

Muv and Vul Mutant Strains of C. elegans

Mutant strains
locus	chr	which is...	Strain	also carries	cell fate needed for	allele	phenotype
let-23	II	receptor tyr kinase	MT2123		req 1 2	n1045	cs Vul
let-60	IV	Ras protein	MT4866		req 1 2	n2021	14% Vul
lin-11	I	LIM homeo-domain	MT633	him-5 (e1647) V	req 2 (some)	n389	Vul
lin-12	III	lin-12/notch type receptor	MT688	unc-32 (e189) III; him-5 (e1647) V	req 2	n941	Vul
lin-15	X	unknown	MT309		req 3	n309	Muv
lin-8; lin-9	II;III		CB1322		synthetic Muv	n111; n112	ts Muv