This page provides an overview of, and links to, established protocols for the sampling, extraction, identification, and culturing of nematodes.

Name	Description
Cryopreservation	This protocols describes how to cryopreserve and revive nematodes. We used this protocols for numerous species.
Frozen nematode preparation for sequencing (bulk)	This protocol is about preparing frozen nematode bulk samples for sequencing.
HMW DNA extraction from frozen nematodes (bulk)	This protocol describes the High Molecular Weight (HMW) DNA extraction from a frozen pellet of nematodes.
Synchronisation	This protocols describes different methods to achieve a synchronised nematode culture.
PiMmS (Picogram input Multimodal Sequencing single - for individuals)	This protocol describes a method for isolating and PCR amplifying low-bias long read (8-16 kb) genomic shotgun libraries from small-bodied invertebrates e.g. for de novo genome assembly.
Barcoding	This protocol is for barcoding nematode species. The primers used in this protocol are tested and work for various genera of nematodes.
Shearing	This protocol describes the shearing of HMW DNA extracted from nematodes using the Megaruptor 3 (Diagenode).
Size selection	This protocol describes the SPRI clean-up with size selection of sheared nematode DNA.
RNA extraction	This protocol describes whole RNA extraction from nematode bulk samples using Trizol.